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For embryo
recovery to be successful it is recommended that the potential donor cow
be.
- At least 90 days fresh,
- in a gaining plane of nutrition, and
- checked by a vet and given a clean bill of
health.
After these criteria have been
met and when a natural heat is observed on the donor, call NorthStar (800.631.3510).
NorthStar will schedule the donor for recovery, starting the program
some where between day 8 and day 13 after the observed heat. Prior to
starting the donor on shots she should be checked by a vet between day
6 and day 8 for a good corpus luteum (CL).
NorthStar sends the producer a shot schedule and the producer administers
shots over a four and one half day period. On the morning of the sixth
day the donor should be in a superovulated heat. She should be bred that
night and given a shot of GnRH and then bred again the next morning. If
she is still showing signs of heat at the second breeding she should then
be bred a third time that evening. If there are any deviations from the
schedule or the donor does not show a good standing heat at the proper
time, call NorthStar.
Recovery
Process:
Seven days after onset of superovulated
heat NorthStar staff will recover the embryos. The donor needs to be confined
for this procedure (head chute, or tied in a comfort stall). The actual
recovery takes about 45 minutes. Finding the embryos in the solution
is the next step and this requires a clean, warm room with an electrical
outlet. This procedure, which uses a microscope takes about 1 1/2 hours.
At this point the embryos can be directly transferred into recipients,
or be prepared for freezing and frozen at the farm.
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